Professional Stem Cell Media

产品
  • 产品
搜索

干细胞培养基供应商

弗元生物-专业的干细胞培养基 >> 资 料 >>产品引用 >>2022年 >> Shen J,Overexpression of PYGO1 promotes early cardiac lineage development in human umbilical cord mesenchymal stromal/stem cells by activating the Wnt/β-catenin pathway
详细内容

Shen J,Overexpression of PYGO1 promotes early cardiac lineage development in human umbilical cord mesenchymal stromal/stem cells by activating the Wnt/β-catenin pathway

Shen J, Wu X, Zhu P, Zhuang J, Qin B, Sun F, Yuan W, Fan X, Jiang Z, Li F, Li Y, Wang Y, Zhao M. Overexpression of PYGO1 promotes early cardiac lineage development in human umbilical cord mesenchymal stromal/stem cells by activating the Wnt/β-catenin pathway. Hum Cell. 2022 Sep 9. doi: 10.1007/s13577-022-00777-3. Epub ahead of print. PMID: 36085540.

Hum Cell. 2022 Sep 9.

 doi: 10.1007/s13577-022-00777-3. Online ahead of print.

Overexpression of PYGO1 promotes early cardiac lineage development in human umbilical cord mesenchymal stromal/stem cells by activating the Wnt/β-catenin pathway

Jie Shen 1Xiushan Wu 2Ping Zhu 3Jian Zhuang 3Bin Qin 2Fang Sun 1Wuzhou Yuan 2Xiongwei Fan 2Zhigang Jiang 2Fang Li 2Yongqing Li 2Yuequn Wang 2Mingyi Zhao 4
 
  • DOI: 10.1007/s13577-022-00777-3

Abstract

Cardiovascular disease still has the highest mortality. Gene-modified mesenchymal stromal/stem cells could be a promising therapy. Pygo plays an important role in embryonic development and regulates life activities with a variety of regulatory mechanisms. Therefore, this study aimed to investigate whether the overexpression of the PYGO1 gene can promote the differentiation of human umbilical cord-derived mesenchymal stromal/stem cells (HUC-MSCs) into early cardiac lineage cells and to preliminary explore the relevant mechanisms. In this study, HUC-MSCs were isolated by the explant method and were identified by flow cytometry and differentiation assay, followed by transfected with lentivirus carrying the PYGO1 plasmid. In PYGO1 group (cells were incubated with lentiviral-PYGO1), the mRNA expressions of cardiac differentiation-specific markers (MESP1, NKX2.5, GATA4, MEF2C, ISL1, TBX5, TNNT2, ACTC1, and MYH6 genes) and the protein expressions of NKX2.5 and cTnT were significantly up-regulated compared with the NC group (cells were incubated with lentiviral-empty vector). In addition, the proportion of NKX2.5, GATA4, and cTnT immunofluorescence-positive cells increased with the inducement time. Overexpression of PYGO1 statistically significantly increased the relative luciferase expression level of Topflash plasmid, the protein expression level of β-catenin and the mRNA expression level of CYCLIND1. Compared with the control group, decreased protein levels of NKX2.5 and cTnT were detected in PYGO1 group after application of XAV-939, the specific inhibitor of the canonical Wnt/β-catenin pathway. Our study suggests that overexpression of PYGO1 significantly promotes the differentiation of HUC-MSCs into early cardiac lineage cells, which is regulated by the canonical Wnt/β-catenin signaling.

Keywords: Cardiac lineage development; Human umbilical cord mesenchymal stromal/stem cells; PYGO1; Wnt/β-catenin signaling.


  • 电话直呼

    • 021-80370389
    • 17621586186
    • 13671816350
    • 13041694656
    • 产品订购1 :
    • 产品订购2 :
    • 技术服务1 :
    • 技术服务2 :
    • 技术服务3 :
  • 微信联系

技术支持: zqjq | 管理登录
seo seo