Liao R, Cao J. Emodin induces osteosarcoma cell apoptosis by promoting ATM protein cleavage[J]. Tropical Journal of Pharmaceutical Research, 2023, 22(1): 31-36.
Abstract
Purpose: To investigate the effect of emodin on osteosarcoma cell proliferation and apoptosis.
Methods: The osteosarcoma cell proliferation ability was determined by CCK-8, apoptosis level and cell
cycle were determined via flow cytometry. Honechst staining was used to determine the ratio of nuclear
pyknosis, Western blotting was used to determine the expression of AKT, p-AKT and caspase-3
precursor protein in each group of osteosarcoma cells.
Results: Emodin significantly inhibited the MG-63 cell proliferation and promoted the apoptosis of
osteosarcoma cells in a dose-dependent manner (p < 0.05). It further blocked osteosarcoma cells in
G1/G0 phase and decreased the expression of AKT, p-AKT, and caspase-3 (p < 0.05).
Conclusion: Emodin inhibits osteosarcoma cell growth by inhibiting ATM protein cleavage. There is a
need to carry out further investigation of the effect of emodin on osteosarcoma cells using animal
models in order to ascertain its potential in the management of osteosarcoma.Keywords: Emodin, ATM, osteosarcoma, Proliferation, Apoptosis