Zhou X, Yuan X, Wang X, et al. Effect and Mechanism of Nystose on Proliferation and Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells[J]. Journal of Biomaterials and Tissue Engineering, 2024, 14(3): 145-151.
DOI: https://doi.org/10.1166/jbt.2024.3364
This study aims to investigate the effect and mechanism of nystose on osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs). hBMSCs were cultured and divided into 5 groups: a control group that received no treatment, and 4 groups treated with varying concentrations of nystose solution (0.5, 1, 5, and 10 μg/mL). The proliferation ability of hBMSCs was tested at different culture times and different concentrations of nystose solution using cell counting kit-8 assay. Alizarin red staining was employed to examine ALP expression and calcium nodule formation in hBMSCs. Western blotting and RT-PCR were conducted to analyze the expressions of alkaline phosphatase (ALP) and Runt-related transcription factor 2 (RUNX2), as well as the levels of c-Jun N-terminal Kinase (JNK)/extracellular regulated protein kinases (ERK) pathway genes. Our findings demonstrated that increasing nystose concentration enhanced cell proliferation. Notably, compared to the control group, nystose intervention significantly elevated ALP activity and expression of osteoblast-related genes. Additionally, it was observed that nystose intervention increased phosphorylation levels of JNK1 and ERK1/2. Conversely, inhibition of JNK/ERK pathway activity reduced ALP and RUNX2 expression and calcium nodule formation, thereby inhibiting osteogenic differentiation of hBMSCs. Nystose intervention effectively regulated the osteogenic differentiation ability of hBMSCs through modulation of the JNK/ERK pathway. These results provide evidence supporting the promotion of osteogenic differentiation of hBMSCs by nystose, thus offering a promising therapeutic approach for bone-related diseases such as osteoporosis.