Authors: Li, Rong; Yue, Juan; Song, Qi; He, Haiyan

Source: Materials Express, Volume 13, Number 7, July 2023, pp. 1154-1162(9)

Publisher: American Scientific Publishers

DOI: https://doi.org/10.1166/mex.2023.2459

This experiment assessed the effect of miR-375 antagonist (mA) modified ferroferric oxide nanoparticles (FONPs) on ovarian cancer cells. SKOV 3 cells were assigned into blank group (normal culture SKOV 3 cells), control group (intervention with FONPs), and intervention group (mA-FONPs), followed by analysis of cell biological behaviors and expressions of Bax, Bcl-2, Caspase-3, E-cadherin, N-cadherin, Vimentin, TL-6, JAK2, and STAT3. The nanoparticles were spherical with excellent dispersion and about 77 nm. Compared with the other two groups, the intervention group showed decreased cell vitality, increased apoptosis (P <0.05). Cell number (44.63+2.37)% and migration quantity (89.75+4.01)% decreased significantly after intervention (P <0.05) along with higher levels of E cadherin, Bax, Caspase 3 activity and lower levels of Bcl-2, N-cadherin, Vimentin, IL-6, JAK2 and STAT3 (P <0.05). miR-375 targeted and inhibited the activity of JAK2/STAT3 pathway, reducing levels of IL-6, p-JAK2, and p-STAT3, up-regulating the expression of Bax and Caspase-3, reducing levels of N-cadherin and Vimentin, and finally regulating cell apoptosis and inhibiting cell migration and invasion.